Phycoerythrin is a fluorescent protein isolated from a red algae.
Keyword:
fluorescent
Keyword:
red algae
Miscellaneous comments:
DATA USED IN REFINEMENT.
RESOLUTION RANGE HIGH (ANGSTROMS) : 2.25;
RESOLUTION RANGE LOW (ANGSTROMS) : 999.00;
DATA CUTOFF (SIGMA(F)) : 0.000;
COMPLETENESS FOR RANGE (%) : 94.6;
CROSS-VALIDATION METHOD : FREE R;
FREE R VALUE TEST SET SELECTION : RANDOM BY LAYERS
Layman's explanation:
Phycoerythrin is a protein isolated from Gastroclonium coulteri, a red algae.
This protein is in part responsible for the red color of the algae. The
molecular weight of this protein is 240,000 daltons, and its composition is
(alpha beta)6gamma. The alpha- and beta- subunits have a molecular weight of
near 20,000 daltons, and the gamma-subunits are approximately 30,000 daltons.
The protein s maximum absorption is 566 nm, and the emission maximum is 575
nm. There are also secondary absorption peaks at 496 nm and 545 nm.
Phycoerythrin is used as a tool in analytical chemistry. The main use of the compound is as a
fluorescent agent; it is one of the most common fluorescent dyes for FACS (fluorescent aromatic compounds). Due
to a large absorption coefficient and high quantum efficiency, it is one of the
brightest dyes in use. It is also used to detect the presence of
cyanobacteria, allowing the estimation of biomass and nitrogen fixation,
identification of phytoplankton (categorization and detection of toxic taxa),
and drinking water quality control.
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