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Thiocarbohydrazide

Thiocarbohydrazide is a common reagent in Transmission and Scanning Electron Microscopy.

Chemical Formula: CH₆N₄S

Layman's explanation: Thiocarbohydrazide (TCH) was discovered in 1908 by R. Stolle. Wilson and Gula were the first to begin studies on this new molecule. Audrieth reinvestigated and improved the original synthesis of TCH and Sandstrom and Beyer were among the first to use TCH in heterocyclic synthesis. TCH has several uses, but the major use is in Transmission and Scanning Electron Microscopy (TEM and SEM). One reason that TCH is such a versatile reagent is that it is osmiophillic, which enables it to be used in cooperation with many osmium reagents, especially and most commonly osmium tetroxide (OsO4). Another reason TCH is commonly used, is because it has an indefinite shelf life, due to its purification process.

Keywords: Scanning Electron Microscopy, Transmission Electron Microscopy, osmiophillic

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